SECOND DAY: Seed Culture Preparation and Sterilization

Date : 5 October 2010

Time : 8.30 a.m

Venue : Laboratory 148

Good morning everyone! We woke up early today because our Mr Fermenter was waiting for us.

Today, we gain a lot of new knowledges...

First thing that we must do is to prepare the seed culture... By using aseptic technique, we transfer the S.cerevisiea into the media that we have prepared yesterday... Then, the seed culture  was leave overnight in the incubator shaker with temperature 37°C...

DONE...!!!

Next step is STERILIZATION.

What is mean by sterilization???

Sterilization is a term referring to any process that eliminates (removes) or kills all forms of life including all transmissible agents such as fungi, bacteria, viruses, spores, etc that present on a surface contain in a fluid, in medication or in a compound such as biological culture media. sterilization can be achieve by applying the proper combinations of heat, chemical, irradiation, high pressure and filtration.

Why the fermenter must be sterilized???

Because we want to prevent the contamination of the media..

Prevention is better than cure right? 

So, what we supposed to do is setting up Mr Fermenter for sterilization...

Here we go...

Picture above... See the tube??? We tighten at the end of each line to prevent the steam to enter the line during sterilization... Make sure, all the inoculation line must be covered. 

The tube was inserted well...

Cover all the end of tube and filter with cotton and aluminium foil.

Make sure all the selected part was covered properly... Do the final check...

Autoclave was ready, waiting for Mr Fermenter to come in...
Autoclave said : welcome fermenter..may you comfortable stay inside me...hehe~

 Locate the fermenter properly.... bye bye fermenter...

Setting up the autoclave...121°C for 15 minutes... Do not prolong the time. It can cause caramelization of media.


After 15 minutes and a few hours to cool down the Mr Fermenter... Then he come out....~

Mr fermenter was successfully sterilized... So, the next step is INOCULATION


Inoculation will be done tomorrow... So, see you tomorrow Mr Fermenter.....
Although the step looks simple, it takes a longer time to setting up the fermenter because a lot of things need to attach there. Therefore, we just see and take pictures as much as we can... 

Oh, tomorrow Mr Sharman will treat us KFC... Waaa, thanks a lot Mr Sharman... We love you..(^_^)