FOURTH DAY

Last Day : WASH.... CLEAN.... WASH....

Date : 7 October 2010

Time : 10.00 a.m

Venue : Lab 148

Today was the last day... What we supposed to do today is CLOSEDOWN AND CLEANING...

Saccharomyces cerevisiae have successfully growth for 1 day. We then ready to clean the yeast and dismantle the fermenter to store as it comes before. That we are using the wild type yeasts those are not pathogenic so we can simply flow the yeast and the medium into sink. It is different if the microbe uses are pathogenic and new type of recombinant. If the microbe are danger to be leave to nature, we have to autoclave it properly just as we autoclave the medium before inoculation. By doing this we manage to kill all the microbe and only release the dead microbe n left over medium to nature. As to be good technologists we have to take care of nature and give no harm to them especially when involving our own research reagent.

Now, WASHING AND CLEANING TIME..!!!!

First, stop any reagent addition, stop aeration and off any remote control from IRIS. Stop the main switches to power supply as well before start to dismantle the component of this fermenter. For our fermentation process the temperature was around room temperature. For other fermentation of microbe that involving high temperature, we need to slow down the temperature and wait until the fermenter are cool down. 

Next step, we empty the reagent bottle lines and discard the cannot reusable reagent. Reagent like antifoam, alkali or acid can be store for next usage.

So, let's have a look how our friend wash the Mr Fermenter..~

All the probes and tubes must be removes...

Next is to clean the vessel, remove the top plate and then remove the probes one by one. As for pH electrodes, after rinse with water need to be cap back into the liquid electrolyte. All the electrodes remove, clean and return to boxes.

Empty the vessel by flowing the medium into the sink...

See... Tadaaaa~ clean and shine enough right????

Observation Under Microscope

We manage to collect some sample before cleaning the fermenter. We drop few drop of sample on slide and observed under microscope. The result seen under the microscope is as below..

S.cerevisiae under microscope.

From the picture above we can clearly see that, the yeast are growth well and there are no contamination happen to the culture. This is one of the benefits of using fermenter to growth culture, the percentage to be contaminated are low. There are difficult to be contaminated unless the inoculums itself have been contaminated before being introduce to the vessel or all the aseptic technique to maintain sterility are not taken.

Finally, our practical class was end... Although it was so tired, but the moment we spent our time with Mr Fermenter, we were more understand what has been thought by Puan Wan Nadiah in our class... We get the overview of using and handling the bioreactor... We can touch it, see it, run it, wash it... And everything... For sure, it was the interesting moment in our life... Not just handling the bioreactor, we also experienced the late night experiment.. That was the most unforgettable moment during the 4 days with Mr Fermenter... Thanks a bunch~ (^_^)